LOCUS       40924_4120       10385 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pAgc-bgyg DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Ikeda Y, Ito K, Izumi Y, Shinomura T.
  TITLE     A candidate enhancer element responsible for high-level expression 
            of the aggrecan gene in chondrocytes
  JOURNAL   J. Biochem. 156 (1), 21-28 (2014)
  PUBMED    24554731
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Ikeda Y, Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and 
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/
REFERENCE   3  (bases 1 to 10385)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10385)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2014"; volume: "156"; issue: "1"; pages: "21-28"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan URL :http://www.tmd.ac.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      17..550
                     /gene="bgyg"
                     /label=rat Agc1 promoter sequence
                     /note="rat Agc1 promoter sequence"
                     /regulatory_class="promoter"
     exon            551..931
                     /gene="bgyg"
                     /note="rat Agc1 exon 1 sequence"
     intron          928..1990
                     /gene="bgyg"
     CDS             2172..5213
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             5229..6263
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     promoter        complement(7254..7272)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7290..7306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7314..7330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7338..7368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7383..7404)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7692..8280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8454..9311)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9312..9416)
                     /label=AmpR promoter
     rep_origin      9750..10205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     10346..10362
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcgaattc gagctcggta ccccaccccc aaaactttcc aacactgttc tgtaagggtt
       61 ctttcaagta gcattcccag ggtagggggt cgtcctgcgc gatcgcatct ttctgggtct
      121 cagctgcaaa tcgcctccag gtatgggggg ggaaccaccc ctccccgcgg ccagggcctg
      181 tcattctgac agcaggagtc gggtgctttc ggcactctgc gggcacagcc ctccggggcc
      241 tcctggactg cggagcttac gaccgcaatg cagacccggg tcctccagcg ctaccagcac
      301 agctttcctc cgcggcccca ggagctgcgg ggtccgcgct cctctcgtgc cctgcgcgcc
      361 cggcgccttc cccagtgagc gcgggtcccc agccctggtc cagagcgctc cggacgtctc
      421 tgccttcccc tccccctgcg aactggcccc gggggtgggg tttccctgtg cgctcgcccc
      481 cacccctagt ttgtgccctc ccctcccccg cccgccccta tgtatgtgtc accgcgcacc
      541 attcccgccc acctacctcc ccgcggtgcc agaggggctc acagagctga ggacgcgtgc
      601 agtaccgccc aaggtccctc attctccgcg actagcatct gacacatgtg ccgaaggaac
      661 tcaagacacc tttctgtatc ccttccctcc atcgaccctg ctccgaaacc cagccagcca
      721 gctaccactg cggccaccgc ccgaggggac ttgcggagga gacgccggcg ggaggagggc
      781 ttcgcagccc ccacccccac ccccactccc agcccagagc atccctgctg cagcgcagca
      841 agacccgggg ctgccagccc ccaggaatcc ctagctgctt agcagggata acggactgaa
      901 gttcttggag gagcgagttc aactcttcaa ggtaaagaaa gggctccttc tctccagagg
      961 agctctgtcc cagggcatct gggaatgctg ctgaggacgc gcgcctaagt gctgggggtg
     1021 gcgtgccttg gtgcccacat tgagtatgtg tgttggggac tcaggacatt agctgcatgc
     1081 ccatggaagt tgagcaaact caactgctgc cccgactcaa agtgaatggg aacacgtgga
     1141 ggggctcctg ctcagagtac acagaattaa gaaagaccag agctttttca agtttaaagt
     1201 accggggcgc taaactgaag tgtagagcga gaaacagttt taaagccaag gaaatgtgtg
     1261 ttcaaacccc actccccccc gcaacccctc gttcctctgg gcgctccata aggctttcat
     1321 attctcttgg gaaggccaac tgatcagaga atcagcaaaa ttatgtcgag ggggggcccg
     1381 gtacccgggg atcccccggg ctgcagagac tcccaggagg ccaaggcagc tggctgcaca
     1441 tactcagttt gaagctccac atgttcaagc cccaggaaaa gctgatgcct caggtcaagt
     1501 ccaaagggag gagaaagtca gcgggaaggc catcagggaa gaagtctctc tccctccaca
     1561 gagggtcctt ttttgtttaa agccttcatg ggggtggctg tgacccaccc acactgaaaa
     1621 gggaggccat tctaggtcta cagactccag agtttatgca aaacccctca cagatataga
     1681 agagagcata aacctgagca tctatggtcc gcctgtcaca tatcactggc taccccagga
     1741 actcattagg atgagtctcc actggctagg agatggggga accagtacac cagccatctt
     1801 ggaattaagc taagaaaggt cgaaggtttc tgaggaaagg attcttagtg aaaacactgt
     1861 ccaaaccatg acaacccgct gttgtgcaat gtgacttcag agccttgggc atgaatttta
     1921 tcactttttt ttcttccttt aactatcccg tgctttaact gtccaaataa catccctgtt
     1981 gtctcttcca gctgaactct gtcgacggta tcgataagct tggatcccca gcttggggga
     2041 tcgaaagagc ctgctaaagc aaaaaagaag tcaccatgtc gtttactttg accaacaaga
     2101 acgtgatttt cgttgccggt ctgggaggca ttggtctgga caccagcaag gagctgctca
     2161 agcgcgatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2221 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2281 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg
     2341 caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg
     2401 tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct
     2461 atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc
     2521 tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg
     2581 gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca
     2641 gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg
     2701 cggtgatggt gctgcgttgg agtgacggca gttatctgga agatcaggat atgtggcgga
     2761 tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt
     2821 tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc
     2881 agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa
     2941 cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt
     3001 atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa
     3061 tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag
     3121 cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga
     3181 acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc
     3241 aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta
     3301 acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct
     3361 acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc
     3421 gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc
     3481 agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg
     3541 gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc
     3601 agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc
     3661 gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt
     3721 cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca
     3781 gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg
     3841 gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt
     3901 ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg
     3961 gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc
     4021 agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc
     4081 gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa
     4141 gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac
     4201 taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg
     4261 cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa
     4321 acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa
     4381 tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc
     4441 tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca
     4501 cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg
     4561 cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt
     4621 gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc
     4681 aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg
     4741 cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga
     4801 actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa
     4861 actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca
     4921 tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga
     4981 attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac
     5041 agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga
     5101 atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg
     5161 cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caggggatcc
     5221 tcgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     5281 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     5341 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     5401 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     5461 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     5521 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     5581 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     5641 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     5701 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     5761 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     5821 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     5881 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     5941 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     6001 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     6061 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     6121 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     6181 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
     6241 atcgggagat gggggaggct aactgacccg ggactagtcg acctgcagcc aacacctaca
     6301 agcacaggct acagaagcgg agcatgagac ccacacggag gagccgcccc agcatggccc
     6361 actgagagga gcttccacaa tgtgcccagg atgctgagcc cagcggccag ccatgctgac
     6421 cgtgcatccc actcacatgg tgtcttcttg tcgctttttg tcatataagg aatccattaa
     6481 agaaggaaaa aaaagccaca ttgtgtgtgc cccactcccc atatcaccca aactgcatct
     6541 aatttatcca ccaccatcac cgccccccat aaaaaaccgc caaagcaaat tcactgtaac
     6601 ccatggactg agtttagaga catttctttc atttcccacc gtgcctttcc agggaccagt
     6661 gcagggacag ggtgagaaga gttaaattaa ataaagaaga ttattttttg ttgttgcttc
     6721 ctgactttac ccaagaacag cacaattgtt ggttacttca cctccaggga gagctagggg
     6781 ccagaggtgg aattggaagg agacagggcc agggagagag gagtagggca gccacacaga
     6841 ccagagcttt aaggactcag aagaggcaaa ctatagctta gtgtgttgga caaaagggat
     6901 cgaggtctgt gccatctatg tgggaaggag cctggggcag ggggcaggct ggccaaaggg
     6961 aagtgaagtt gcctttcctc cagggcccct ctcctaggaa cctttctcct aggaaccttt
     7021 cttctgggtc aaccacccta gcaggtgtca tttctcggct ggactgggta ggggcacttc
     7081 cttcccagga gtgctccgcc gtccccttcc ctctccccac cccgggacgg tgcaggcacc
     7141 agtgttccgt gcacctattt atatttttga aaactaaaga ttatgataat tataataata
     7201 aagacattgg aagagatctg ctagctcgag aattcgatat caagcttgag tattctatag
     7261 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7321 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7381 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7441 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7501 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7561 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7621 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7681 ttgctggcgt ttttcgatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7741 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7801 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7861 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7921 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7981 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8041 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8101 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8161 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8221 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8281 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8341 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8401 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8461 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8521 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8581 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8641 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8701 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttggc
     8761 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8821 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8881 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8941 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9001 gagtactcaa ccaagtcatt ctgagaatac cgcgcccggc gaccgagttg ctcttgcccg
     9061 gcgtcaatac gggataatag tgtatgacat agcagaactt taaaagtgct catcattgga
     9121 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9181 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9241 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9301 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9361 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9421 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9481 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     9541 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9601 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     9661 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     9721 tgcgtaagga gaaaataccg catcaggcga cgcgccctgt agcggcgcat taagcgcggc
     9781 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9841 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9901 tcgggggctc cctttagggt tccgatttag agctttacgg cacctcgacc gcaaaaaact
     9961 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    10021 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    10081 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    10141 aaaaaatgag ctgatttaac aaatatttaa cgcgaatttt aacaaaatat taacgtttac
    10201 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    10261 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    10321 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
    10381 ctata
//
